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Merck KGaA hanging cell culture inserts millicell
Hanging Cell Culture Inserts Millicell, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/millicell+hanging+cell+culture+inserts/millicell+hanging+cell+culture+inserts/pmc12218338-318-9-13
Average 90 stars, based on 1 article reviews
hanging cell culture inserts millicell - by Bioz Stars, 2026-10
90/100 stars

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Cell Culture:

Article Title: Cancer‐testis gene STK31 is regulated by methylation and promotes the development of pancreatic cancer
Article Snippet: .. Twenty‐four well Millicell Hanging Cell Culture Inserts (Merck Millipore) and Biocoat Matrigel (BD Bioscience) were used to access the cell migration and invasion ability. ..

Article Title: Optimization of the spherical integrity for sustained-release alginate microcarriers-encapsulated doxorubicin by the Taguchi method
Article Snippet: .. Literally, ~ 0.3 g microcarriers were placed onto MILLICELL hanging cell culture inserts (Merck KGaA, Darmstadt, Germany) in 12-well plates. ..

Article Title: Metallothionein 1M (MT1M) inhibits lung adenocarcinoma cell viability, migration, and expression of cell mobility-related proteins through MDM2/p53/MT1M signaling
Article Snippet: .. After exposure to various treatments and growth conditions, the cells were suspended in F-12K medium without FBS supplementation and seeded into the upper chamber of Millicell hanging cell culture inserts (Merck Millipore, Darmstadt, Germany). ..

Article Title: Toxoplasma gondii eIF-5A Modulates the Immune Response of Murine Macrophages In Vitro.
Article Snippet: For migration assays, 5 × 106 Ana-1 macrophages were stimulated with rTgeIF-5A proteins at concentrations of 0, 5, 10, 20, 40 or 80 μg/mL or pET-32a vector protein for 24 h in a 24-well plate (Corning Costar, Cambridge, MA, USA), respectively. .. The cocultured cells were inserted into Millicell Hanging Cell Culture Inserts (Merck Millipore, Billerica, MA, USA) and allowed to migrate toward monocyte chemoattractant protein (MCP-1 or CCL2, Medchemexpress, Shanghai, China) or no chemokines for 24 h. The migrated cells were counted under a light microscope, and the migration rate was analyzed. .. Ana-1 cell suspensions were seeded in 12-well plates (Corning Costar, Cambridge, MA, USA) and incubated with rTgeIF-5A proteins at concentrations of 0, 5, 10, 20, 40 or 80 μg/mL, pET-32a vector protein or PBS for 24 h in a CO2 incubator at 37 ◦C, respectively.

Article Title: Sustained-Release and pH-Adjusted Alginate Microspheres-Encapsulated Doxorubicin Inhibit the Viabilities in Hepatocellular Carcinoma-Derived Cells
Article Snippet: .. Specifically, 2 × 10 5 microspheres, calculated by an automated cell counter (TC20, Bio-Rad, Hercules, CA, USA), were placed onto Millicell ® hanging cell culture inserts (Merck KGaA, Darmstadt, Germany) in 48-well plates. ..

Article Title: Toxoplasma gondii eIF-5A Modulates the Immune Response of Murine Macrophages In Vitro
Article Snippet: For migration assays, 5 × 10 6 Ana-1 macrophages were stimulated with r Tg eIF-5A proteins at concentrations of 0, 5, 10, 20, 40 or 80 μg/mL or pET-32a vector protein for 24 h in a 24-well plate (Corning Costar, Cambridge, MA, USA), respectively. .. The cocultured cells were inserted into Millicell Hanging Cell Culture Inserts (Merck Millipore, Billerica, MA, USA) and allowed to migrate toward monocyte chemoattractant protein (MCP-1 or CCL2, Medchemexpress, Shanghai, China) or no chemokines for 24 h. The migrated cells were counted under a light microscope, and the migration rate was analyzed. .. Ana-1 cell suspensions were seeded in 12-well plates (Corning Costar, Cambridge, MA, USA) and incubated with r Tg eIF-5A proteins at concentrations of 0, 5, 10, 20, 40 or 80 μg/mL, pET-32a vector protein or PBS for 24 h in a CO 2 incubator at 37 °C, respectively.

Article Title: PDZRN4 -mediated colon cancer cell proliferation and dissemination is regulated by miR-221-3p.
Article Snippet: .. Cells were seeded to the upper chamber of Millicell hanging cell culture inserts (Merck Millipore, Darmstadt, Germany). ..

Article Title: Photorhabdus luminescens Tc toxin is inhibited by the protease inhibitor MG132 and activated by protease cleavage resulting in increased binding to target cells.
Article Snippet: .. For the TER assay, CaCo-2 cells were plated on Millicell hanging cell culture inserts (Merck Chemicals, Darmstadt, Germany) and incubated 5-7 days with medium exchange every 3 days. ..

Migration:

Article Title: Cancer‐testis gene STK31 is regulated by methylation and promotes the development of pancreatic cancer
Article Snippet: .. Twenty‐four well Millicell Hanging Cell Culture Inserts (Merck Millipore) and Biocoat Matrigel (BD Bioscience) were used to access the cell migration and invasion ability. ..

Article Title: Toxoplasma gondii eIF-5A Modulates the Immune Response of Murine Macrophages In Vitro.
Article Snippet: For migration assays, 5 × 106 Ana-1 macrophages were stimulated with rTgeIF-5A proteins at concentrations of 0, 5, 10, 20, 40 or 80 μg/mL or pET-32a vector protein for 24 h in a 24-well plate (Corning Costar, Cambridge, MA, USA), respectively. .. The cocultured cells were inserted into Millicell Hanging Cell Culture Inserts (Merck Millipore, Billerica, MA, USA) and allowed to migrate toward monocyte chemoattractant protein (MCP-1 or CCL2, Medchemexpress, Shanghai, China) or no chemokines for 24 h. The migrated cells were counted under a light microscope, and the migration rate was analyzed. .. Ana-1 cell suspensions were seeded in 12-well plates (Corning Costar, Cambridge, MA, USA) and incubated with rTgeIF-5A proteins at concentrations of 0, 5, 10, 20, 40 or 80 μg/mL, pET-32a vector protein or PBS for 24 h in a CO2 incubator at 37 ◦C, respectively.

Article Title: Toxoplasma gondii eIF-5A Modulates the Immune Response of Murine Macrophages In Vitro
Article Snippet: For migration assays, 5 × 10 6 Ana-1 macrophages were stimulated with r Tg eIF-5A proteins at concentrations of 0, 5, 10, 20, 40 or 80 μg/mL or pET-32a vector protein for 24 h in a 24-well plate (Corning Costar, Cambridge, MA, USA), respectively. .. The cocultured cells were inserted into Millicell Hanging Cell Culture Inserts (Merck Millipore, Billerica, MA, USA) and allowed to migrate toward monocyte chemoattractant protein (MCP-1 or CCL2, Medchemexpress, Shanghai, China) or no chemokines for 24 h. The migrated cells were counted under a light microscope, and the migration rate was analyzed. .. Ana-1 cell suspensions were seeded in 12-well plates (Corning Costar, Cambridge, MA, USA) and incubated with r Tg eIF-5A proteins at concentrations of 0, 5, 10, 20, 40 or 80 μg/mL, pET-32a vector protein or PBS for 24 h in a CO 2 incubator at 37 °C, respectively.

Light Microscopy:

Article Title: Toxoplasma gondii eIF-5A Modulates the Immune Response of Murine Macrophages In Vitro.
Article Snippet: For migration assays, 5 × 106 Ana-1 macrophages were stimulated with rTgeIF-5A proteins at concentrations of 0, 5, 10, 20, 40 or 80 μg/mL or pET-32a vector protein for 24 h in a 24-well plate (Corning Costar, Cambridge, MA, USA), respectively. .. The cocultured cells were inserted into Millicell Hanging Cell Culture Inserts (Merck Millipore, Billerica, MA, USA) and allowed to migrate toward monocyte chemoattractant protein (MCP-1 or CCL2, Medchemexpress, Shanghai, China) or no chemokines for 24 h. The migrated cells were counted under a light microscope, and the migration rate was analyzed. .. Ana-1 cell suspensions were seeded in 12-well plates (Corning Costar, Cambridge, MA, USA) and incubated with rTgeIF-5A proteins at concentrations of 0, 5, 10, 20, 40 or 80 μg/mL, pET-32a vector protein or PBS for 24 h in a CO2 incubator at 37 ◦C, respectively.

Article Title: Toxoplasma gondii eIF-5A Modulates the Immune Response of Murine Macrophages In Vitro
Article Snippet: For migration assays, 5 × 10 6 Ana-1 macrophages were stimulated with r Tg eIF-5A proteins at concentrations of 0, 5, 10, 20, 40 or 80 μg/mL or pET-32a vector protein for 24 h in a 24-well plate (Corning Costar, Cambridge, MA, USA), respectively. .. The cocultured cells were inserted into Millicell Hanging Cell Culture Inserts (Merck Millipore, Billerica, MA, USA) and allowed to migrate toward monocyte chemoattractant protein (MCP-1 or CCL2, Medchemexpress, Shanghai, China) or no chemokines for 24 h. The migrated cells were counted under a light microscope, and the migration rate was analyzed. .. Ana-1 cell suspensions were seeded in 12-well plates (Corning Costar, Cambridge, MA, USA) and incubated with r Tg eIF-5A proteins at concentrations of 0, 5, 10, 20, 40 or 80 μg/mL, pET-32a vector protein or PBS for 24 h in a CO 2 incubator at 37 °C, respectively.

Incubation:

Article Title: Photorhabdus luminescens Tc toxin is inhibited by the protease inhibitor MG132 and activated by protease cleavage resulting in increased binding to target cells.
Article Snippet: .. For the TER assay, CaCo-2 cells were plated on Millicell hanging cell culture inserts (Merck Chemicals, Darmstadt, Germany) and incubated 5-7 days with medium exchange every 3 days. ..



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